Silhueta de pessoa indiferenciada

Manuel Aureliano Pereira Martins Alves

Professor associado com agregação
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Docentes Universitário
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Exclusividade
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CT em Funções Públicas por tempo indeterminado
Manuel Aureliano Pereira Martins Alves, residente em Faro, nasceu no concelho de Gondomar, Distrito do Porto, filho de Manuel Martins Alves e de Maria Rosa Alves Pereira. Licenciado em Bioquímica, mestre em Biologia Celular e doutor em Bioquímica (especialidade em Bioenergética) pela Universidade de Coimbra. Atualmente é Professor Associado na FCT, com Agregação em Bioquímica Inorgânica, Universidade do Algarve, onde é responsável pelas disciplinas de Bioquímica I, Bioquímica, Bioquímica Inorgânica e Química/Bioquímica Avançada a cursos de licenciatura (BQ, BT, CBM) e de mestrado (MICF e Biotecnologia). Investigador no CCMar onde formou o grupo de BioVanádio, posteriormente designado por Bioquímica Celular e Inorgânica. Manuel Aureliano Alves, autor e/ou co-autor de 112 publicaçoes científicas, 227 comunicaçoes orais e em poster, 55 palestras em escolas do ensino básico e secundário, participou em 194 júris e foi orientador e/ou co-orientador de 100 alunos. Revisor de 626 artigos científicos em de 96 revistas, tendo sido destacado por esta atividade na plataforma Publons (Top 1%), tendo ainda sido nomeado na lista dos "Top 10 reviewers" da revista Metallomics durante 3 anos consecutivos (2018, 2019 e 2020). Diretor e/ou membro da comissão de curso da licenciatura em Bioquimica (UAlg) durante 15 anos, promoveu desde 1997/98 a formação, a divulgação e a investigação em bioquímica na Universidade do Algarve, tendo participado em 17 projectos de investigação e/ou divulgação das ciências na escolas. Investigador do CCMar desde 2006, membro da direcção da sociedade portuguesa de bioquímica (SPB) desde 2012, onde tem promovido a investigação e a divulgação da bioquímica em Portugal. Especialista mundial em estudos sobre ¿decavanadato¿ e ¿vanadato¿, tem actualmente como principais tópicos de investigação o estudo dos mecanismos de acção de polioxometalatos e de complexos de metais com propriedades antidiabéticas, anticancerígenas e antibacterianas, no âmbito de projectos e/ou colaborações nacionais (Lisboa, Algarve, Leiria, Évora, Coimbra, Aveiro) e internacionais com grupos de investigação, por exemplo, da Austria, Índia, USA, México, Suécia, Australia, Eslováquia e Espanha. Recentemente foi incluído no "World¿s Top 2% Scientists list¿ dos cientistas mais influentes do mundo, cujos trabalhos de investigação publicados aceleraram o progresso nas suas respectivas áreas e influenciaram a produtividade do trabalho de outros investigadores: 1) "Impacto-carreira" e 2) "Impacto ano de 2020" (https://fct.ualg.pt/17-cientistas-da-ualg-encontram-se-entre-os-mais-citados-nivel-mundial).

Atividades

Atividades
2021/10/15. Apresentação oral de trabalho. Polyoxovanadates biological activities: the present comes from the future! Manuel Aureliano, Universidade do Algarve,. V12 Vanadium Symposium. Cyprus.
2020. Apresentação oral de trabalho. Aplicaçoes biológicas de polioxometalatos. Departamento de Química. Universidade Nova de Lisboa (Caparica, Portugal).
2019. Apresentação oral de trabalho. 35 anos a dançar com o decavanadato: interação com proteínas versus estudos in vivo e in vitro!. XX anos do curso de Bioquímica, 2019. Uni Evora (Évora, Portugal).
2019. Apresentação oral de trabalho. Polyoxovanadates with antibacterial activity: Are P-type ATPases the only target? . ICBIC-19 19th International Conference on Biological Inorganic Chemistry 2019.
2019. Apresentação oral de trabalho. Polyoxotungstates with anticancer activity: Are membrane proteins attractive targets? . 10th Meeting on Signal transduction - Sinal 2019.
2019. Apresentação oral de trabalho. Proposed mechanisms of the anticancer activity of polyoxometalates. 10th Meeting on Signal transduction - Sinal 2019.
2018. Apresentação oral de trabalho. Os cursos de Bioquímica em Portugal. XXV Aniversário do Curso de Bioquímica no Algarve.
2018. Apresentação oral de trabalho. Thirty years of dancing with decavanadate: From chemistry to “in vivo” studies!. 11th International Vanadium Symposium.
2018. Apresentação oral de trabalho. Vanadium applications, toxicity mechanisms and allergic reactions. VIII Workshop em Bioquímica Clínica .
2018. Apresentação oral de trabalho. Recent Insights Into the Biological Activities of Polyoxometalates. 24th IUPAC International Conference on Physical Organic Chemistry. Universidade do Algarve (Faro, Portugal).
2018/01/01 - 2018/12/31. Arbitragem científica em revista. Metallomics.
2017. Apresentação oral de trabalho. "30th years of dancing with decavanadate” Part I: Interactions with proteins. Fakultät für Chemie, Institut für Biophysikalische Chemie. Universität Wien (Viena, Áustria).
2017. Apresentação oral de trabalho. "30th years of dancing with decavanadate” Part II: In vivo and in vitro studies. Fakultät für Chemie, Institut für Biophysikalische Chemie. Universität Wien (Viena, Áustria).
2017. Apresentação oral de trabalho. New insights into protein interactions of decavanadate. Department of Inorganic Chemistry. University of Bratislava (Bratislava, Eslováquia).
2017. Apresentação oral de trabalho. A brief overview of the Biochemistry degrees in Portugal. Meeting of Coordinators of Bachelor and Master degrees in Biochemistry, FEBS3+.
2017. Apresentação oral de trabalho. New insights into actin interactions of decavanadate: CD Spectroscopic Studies. 2nd International Caparica Christmas Congress on Translational Chemistry.
2017. Apresentação oral de trabalho. POMs as inhibitors of P-type ATPases and the role of polyphenols. 11th World Congress on Polyphenols Applications.
2017/01/01 - 2017/12/31. Arbitragem científica em revista. Metallomics.
2016. Apresentação oral de trabalho. Celebrating Decavanadate (V10) at the 10th International Vanadium Symposium. The 10th International Vanadium Symposium.
2016. Apresentação oral de trabalho. Isostructural decavanadate and decaniobate decompose into different oligomers: biochemical relevance. 1º Encontro Nacional de Bioinorgânica.

Projetos

Projetos
2011/01/01 - 2016/12/31. 2011-2015, Projecto PTDC/QUI-BIQ/112943/2009, Caracterização da polaridade em bicamadas lipidicas de fosfolipidos e colesterol e dos seus efeitos em enzimologia menbranar.. Investigador.
2005/01/01 - 2009/12/31. 2005/2008- Projecto POCI/SAU-MMO/57598/2004, Estudo dos possiveis factores ambientais e moleculares que levam ao desenvolvimento de diabetes tipo 2 e obesidade em Portugal (Study of the possible environmental and molecular mechanisms responsible for Insulin Resistance, Type 2 Diabetes and Obesity in. Investigador.
2005/01/01 - 2007/12/31. 2005/07, Projecto Ciencia Viva VI, PVI-1458, Metais por todo o lado, FCT-UALG/Escola D. Afonso III, Faro. Membro da equipa e coordenador científico-pedagógico.. Investigador.
2005/01/01 - 2006/12/31. 2005/2006, Projecto CRUP, E-106/05. “Oxidative stresse in the contractile system induced by metal ions” em colaboração com Dr Carlos Merino, Univ. Extremadura. Investigador.
2002/01/01 - 2005/12/31. POCT/QUI/38191/2001-“Cadmium and vanadium compounds interactions with calcium pump. Investigador responsável.
2001/01/01 - 2005/12/31. 2001/2005, Projecto Cost D21: “Insulin-mimetic actions of vanadium compounds”. Membro da equipa, (PI) D. Redher, Germany. Investigador.
2001/01/01 - 2004/01/01. 2001/2004, POCTI/BME/34701/99, “Structure and function of elicitins and their role in the cork oak infection by the fungus Phytophora cinnamomi. Investigador.
2000/10 - 2003/09. POCTI/34668/Fis/2000 -“Field effect transition array for monitoring electrical activity from single cell culture.
2000/10/01 - 2003/09/30. POCTI/34668/Fis/2000 -“Field effect transition array for monitoring electrical activity from single cell culture. Investigador.
2000/01 - 2002/12. 2000-2002- Projecto Ciência Viva, PIV-041 “A química dos metais e as interfaces com as ciências biológicas” (EB 2,3 Afonso III, Faro) Coordenador científico-pedagógico.
2000/01/01 - 2002/12/31. 2000-2002- Projecto Ciência Viva, PIV-041 “A química dos metais e as interfaces com as ciências biológicas” (EB 2,3 Afonso III, Faro) Coordenador científico-pedagógico. Investigador.
1988/01/01 - 1999/12/31. 1998/99, Projecto Ciência Viva, PIII-311, Poluição das Águas e Solos no Concelho de Oliveira do Bairro, EB2,3 Dr Acácio de Azevedo, Oliveira do Bairro, Coordenador científico-pedagógico.. Investigador.
1998/01/01 - 1999/12/31. 1998/99, Projecto Ciência Viva, PIII-101, Influência de Alterações Ambientais nos Seres Vivos - Métodos Laboratoriais Interdisciplinares da Biologia e da Química (EB2,3 Afonso III, Faro) Coordenador científico-pedagógico. Investigador.
1994/01/01 - 1998/12/31. Muscle Network Contract ERBCHRXCT-940606, EU, 100.000 ECU,1994-1998.. Investigador.
1997/01/01 - 1998/12/31. 1997/98, Projecto Ciência Viva, PII-223, Poluentes atmosféricos e seus efeitos na saúde: métodos laboratoriais interdisciplinares da biologia e da química (EB2,3 Dr Acácio de Azevedo, Oliveira do Bairro) Ana Lima (Coordenador), M. Aureliano, Coordenador científico-pedagógico.. Investigador.
1997/01/01 - 1998/12/31. 1997/98, Projecto Ciência Viva, PII-311, Conservação e Valorização do patrimonio natural e cultural do Oppidum de Conimbriga e das terras de Sicó", Museu de Conímbriga, Coordenador.. Investigador.
1997/01/01 - 1998/12/31. 1997/98, Projecto Ciência Viva, PII-312, Estudo do Ambiente no Território do Oppidum de Conimbriga/Terras de Sicó, Museu de Conímbriga, Coordenador científico-pedagógico.. Investigador.

Produções

Aureliano, M.. "Decavanadate interactions with sarcoplasmic reticulum calcium pump". Portugal: Research Signpost. http://hdl.handle.net/10400.1/1300
Tiago, Teresa; Gutiérrez-Merino, Carlos; Aureliano, M.. "Monomeric versus decameric vanadate in the elucidation of muscle contraction regulation: a kinetic, spectroscopic and structural overview". Portugal: Research SignPost. http://hdl.handle.net/10400.1/1307
Soares, S. S.; Gutiérrez-Merino, Carlos; Aureliano, M.. "Decavanadate toxicity effects following in vivo administration". Portugal: Research Signpost. http://hdl.handle.net/10400.1/1301
Aureliano, M.. "Biological effects of decavanadate: muscle contraction, in vivo oxidative stress, and mitochondrial toxicity". Portugal: American Chemical Society Symposium series. http://hdl.handle.net/10400.1/1306
Tiago, Daniel M.; Laizé, Vincent; Aureliano, M.; Cancela, Leonor. "Vanadate effects on bone metabolism: fish cell lines as an alternative to mammalian in vitro systems". Portugal: Research Signpost. http://hdl.handle.net/10400.1/1302
Tiago, Teresa; Aureliano, M.; Gutiérrez-Merino, Carlos. "Effects of reactive oxygen and nitrogen species on actomyosin and their implications for muscle contractility". Portugal: Research Signpost. http://hdl.handle.net/10400.1/1311
Manuel Aureliano. 2022. "The Future Is Bright for Polyoxometalates". BioChem. https://doi.org/10.3390/biochem2010002
Alves, Manuel Aureliano Pereira Martins. "Polyoxidovanadates' interactions with proteins: An overview". Coordination Chemistry Reviews, 01 de março, 2022. https://doi.org/10.1016/j.ccr.2021.214344
Manuel Aureliano; Nadiia I. Gumerova; Giuseppe Sciortino; Eugenio Garribba; Annette Rompel; Debbie C. Crans. 2021. "Polyoxovanadates with emerging biomedical activities". Coordination Chemistry Reviews, 447: 214143-214143. https://doi.org/10.1016/j.ccr.2021.214143
Cristina Amante; Ana Luísa De Sousa-Coelho; Manuel Aureliano. 2021. "Vanadium and Melanoma: A Systematic Review". Metals, 11 (5): 828-828. https://doi.org/10.3390/met11050828
Giuseppe Sciortino; Manuel Aureliano; Eugenio Garribba. 2021. "Rationalizing the Decavanadate(V) and Oxidovanadium(IV) Binding to G-Actin and the Competition with Decaniobate(V) and ATP". Inorganic Chemistry, 60 (1): 334-344. https://doi.org/10.1021/acs.inorgchem.0c02971
Maria Berrocal; Juan J. Cordoba-Granados; Sónia A. C. Carabineiro; Carlos Gutierrez-Merino; Manuel Aureliano; Ana M. Mata. 2021. "Gold Compounds Inhibit the Ca2+-ATPase Activity of Brain PMCA and Human Neuroblastoma SH-SY5Y Cells and Decrease Cell Viability". Metals. https://doi.org/10.3390/met11121934
Catarina Pimpão; Inês V. da Silva; Andreia F. Mósca; Jacinta O. Pinho; Maria Manuela Gaspar; Nadiia I. Gumerova; Annette Rompel; Manuel Aureliano; Graça Soveral. 2020. "The Aquaporin-3-Inhibiting Potential of Polyoxotungstates". International Journal of Molecular Sciences. https://doi.org/10.3390/ijms21072467
Custódia Fonseca; Gil Fraqueza; Sónia A. C. Carabineiro; Manuel Aureliano. 2020. "The Ca2+-ATPase Inhibition Potential of Gold(I, III) Compounds". Inorganics, 8 (9): 49-49. https://doi.org/10.3390/inorganics8090049
Fraqueza, Gil; Fuentes, Juan; Krivosudský, Lukáš; Dutta, Saikat; Mal, Sib Sankar; Roller, Alexander; Giester, Gerald; Rompel, Annette; Aureliano, Manuel. 2019. "Inhibition of Na+/K+- and Ca2+-ATPase activities by phosphotetradecavanadate". Journal of Inorganic Biochemistry, 197. https://doi.org/10.1016/j.jinorgbio.2019.110700
Marques-da-Silva, Dorinda; Fraqueza, Gil; Lagoa, Ricardo; Vannathan, Anjana Anandan; Mal, Sib Sankar; Aureliano, Manuel; Dorinda Marques-da-Silva; et al. 2019. "Polyoxovanadate inhibition of Escherichia coli growth shows a reverse correlation with Ca2+-ATPase inhibition". New Journal of Chemistry, 43 (45): 17577-17587. https://doi.org/10.1039/c9nj01208g
Bijelic, Aleksandar; Aureliano, Manuel; Rompel, Annette. 2018. "Im Kampf gegen Krebs: Polyoxometallate als nächste Generation metallhaltiger Medikamente". Angewandte Chemie, 131 (10): 3008-3029. https://doi.org/10.1002/ange.201803868
M. Aureliano; Bijelic, Aleksandar; Aureliano, Manuel; Rompel, Annette. 2018. "Polyoxometalates as potential next-generation metallodrugs in the combat against cancer". Angewandte Chemie International Edition, 58 (10): 2980-2999. https://doi.org/10.1002/anie.201803868
M. Aureliano. 2018. "Peroxynitrite versus decavanadate protein oxidative modifications: the case of myosin".
M. Aureliano. 2018. "Outstanding Reviewers for Metallomics in 2017".
Aleksandar Bijelic; Manuel Aureliano; Annette Rompel. 2018. "The antibacterial activity of polyoxometalates: structures, antibiotic effects and future perspectives". Chemical Communications, 54 (10): 1153-1169. https://doi.org/10.1039/C7CC07549A
Gumerova N; Krivosudský L; Fraqueza G; Breibeck J; Al-Sayed E; Tanuhadi E; Bijelic A; et al. 2018. "The P-type ATPase inhibiting potential of polyoxotungstates.". Metallomics : integrated biometal science, 10 (2): 287-295. https://doi.org/10.1039/c7mt00279c
Aureliano, M.. 2017. The role of decavanadate in anti-tumor activity. Decavanadate compounds were described to be involved in a variety of biological activities and responses such as anti-virus, anti-bacterial and anticancer. While the mechanisms of action of the antiviral and anti-bacterial activities are better understood, the same does not go for the anti-tumour activity. Nevertheless, the inhibition of tumour proliferation seems to impact certain enzymes such as. http://hdl.handle.net/10400.1/10265
M. Aureliano. 2017. "Polyoxometalates as inhibitors of P-type ATPases and the role of polyphenols". ISANH, Vol. 5, Issue 2, 2017. https://doi.org/10.18143/AISANH_v5i2_7
Marques MPM; Gianolio D; Ramos S; Batista de Carvalho LAE; Aureliano M. 2017. "An EXAFS Approach to the Study of Polyoxometalate-Protein Interactions: The Case of Decavanadate-Actin.". Inorganic chemistry. https://doi.org/10.1021/acs.inorgchem.7b01018
Fonseca TG; Morais MB; Rocha T; Abessa DM; Aureliano M; Bebianno MJ. 2017. "Ecotoxicological assessment of the anticancer drug cisplatin in the polychaete Nereis diversicolor.". The Science of the total environment. https://doi.org/10.1016/j.scitotenv.2016.09.185
Aureliano, M.; Ohlin, C. André; Vieira, Michele O.; Marques, M. Paula M.; Casey, William H.; Batista de Carvalho, Luís A. E.; Aureliano M; et al. 2016. Characterization of decavanadate and decaniobate solutions by Raman spectroscopy. The decaniobate ion, (Nb10 = [Nb10O28]6-) being isoelectronic and isostructural with the decavanadate ion (V10 = [V10O28]6-), but chemically and electrochemically more inert, has been useful in advancing the understanding of V10 toxicology and pharmacological activities. In the present study, the solution chemistry of Nb10 and V10 between pH 4 and 12 is studied by Raman spectroscopy. The Raman spe. https://doi.org/10.1039/c5dt04176g
Aureliano, M.. 2016. Decavanadate contribution to vanadium biomarkers. The levels of vanadium in urine and blood can be used as biomarkers of exposure, but the mechanism of vanadium toxicity is of major relevance in order to understand how biomarkes can be valuable. Our research group has performed in vivo and in vitro studies using fish and rat models to analysed and compare the toxicity effects induce by vanadium(V) species in the forms of vanadate (V1) and decavan. https://doi.org/10.4172/2471-8556.C1.003
Aureliano, M.; Aureliano M. 2016. Decavanadate toxicology and pharmacological activities: V10 or V1, both or none?. This review covers recent advances in the understanding of decavanadate toxicology and pharmacological applications. Toxicological in vivo studies point out that V10 induces several changes in several oxidative stress parameters, different from the ones observed for vanadate (V1). In in vitro studies with mitochondria, a particularly potent V10 effect, in comparison with V1, was observed in the mi. https://doi.org/10.1155/2016/6103457
Custódia Fonseca; Manuel Aureliano; Feras Abbas; Ambrose Furey; Fonseca, C.; Aureliano, M.; Abbas, F.; Furey, A.. 2015. Recent insights into anatoxin-achemical synthesis, biomolecular targets, mechanisms of action and LC-MS detection. https://doi.org/10.1002/9781118500354.ch7
Pereira, Maria João; Aureliano, M.; Palming, Jenny; Rizell, Magnus; Carvalho, Eugénia; Svensson, Maria K.; Eriksson, Jan W.; et al. 2014. Cyclosporine A and tacrolimus reduce the amount of GLUT4 at the cell-surface in human adipocytes: increased endocytosis as a potential mechanism for the diabetogenic effects of immunosuppressive agents. Context:Immunosuppressive agentes are associated with profound metabolic side effects including new-onset diabetes and dyslipidemia after organ transplantation. Objective: Toi nvestigated the effects of the cyclosporine A(CsA)or tacrolimus ong lucose uptake and insulin signalling in human adipocytes and their impact on the regulation of celular trafficking of the glucose transporter 4 (GLUT4). Des. https://doi.org/10.1210/jc.2014-1266
Aureliano, M.. 2014. Metais na bioquímica e na medicina. A Bioquímica ou Química da Vida representa uma ciência interdisciplinar que utiliza estratégias e métodos de todas as Ciências Exactas e Naturais. Nos últimos 10 anos, catorze prémios Nobel da Química, Fisiologia e Medicina foram atribuídos na área da Bioquímica o que reflecte a importância desta área de conhecimento nas Sociedades contemporâneas. A Bioquímica não se reduz apenas ao estudo dos com. http://hdl.handle.net/10400.1/4897
Aureliano, M.. 2014. A química da vida. A Bioquímica ou Química da Vida é uma ciência interdisciplinar que utiliza estratégias e métodos de todas as Ciências Exactas e Naturais. Nos últimos 10 anos, foram catorze os prémios Nobel da Química, Fisiologia e Medicina que foram atribuídos na área da Bioquímica o que reflecte a importância desta área de conhecimento nas Sociedades contemporâneas. A Química da Vida não se reduz apenas ao estud. http://hdl.handle.net/10400.1/4882
Aureliano, M.. 2014. "A bioquímica na sociedade".
Aureliano, M.; Samart, Nuttaporn; Saeger, Jessica; Haller, Kenneth J.; Crans, Debbie C.. 2014. Interaction of decavanadate with interfaces and biological modelmembranesystems:Haracterization of soft oxometale systems. Decavanadate is a polyoxometalate consisting of 10 octahedral vanadium centers, which has been found to exert biological effects and has been observed in vivo. Biological activity implies that a material is taken up into a cell or that the material interacts with membrane receptors. Because of the large size and the high molecular charge, it is nontrivial to anticipate how such a large anion inter. https://doi.org/10.1142/S2251237314400073
NUTTAPORN SAMART; JESSICA SAEGER; KENNETH J. HALLER; MANUEL AURELIANO; DEBBIE C. CRANS. 2014. "INTERACTION OF DECAVANADATE WITH INTERFACES AND BIOLOGICAL MODEL MEMBRANE SYSTEMS: CHARACTERIZATION OF SOFT OXOMETALATE SYSTEMS". Journal of Molecular and Engineering Materials, 02 (01): 1440007-1440007. https://doi.org/10.1142/s2251237314400073
Aureliano, M.. 2014. "Decavanadate contribution to vanadium biochemistry: In vitro and in vivo studies". Inorganica Chimica Acta, 420: 4-7. https://doi.org/10.1016/j.ica.2013.10.010
Aureliano, M.; Ohlin, C. Andre. 2014. "Decavanadate in vitro and in vivo effects: facts and opinions". Journal of Inorganic Biochemistry, 137: 123-130. https://doi.org/10.1016/j.jinorgbio.2014.05.002
Aureliano, M.. 2013. Ion pumps as biological targets for decavanadate. The putative applications of poly-, oligo- and mono-oxometalates in biochemistry, biology, pharmacology and medicine are rapidly attracting interest. In particular, these compounds may act as potent ion pump inhibitors and have the potential to play a role in the treatment of e.g. ulcers, cancer and ischemic heart disease. However, the mechanism of action is not completely understood in most cases. http://hdl.handle.net/10400.1/2699
Pereira, Maria João; Palming, Jenny; Rizell, Magnus; Aureliano, M.; Carvalho, Eugénia; Svensson, Maria K.; Eriksson, Jan W.; et al. 2013. The immunosuppressive agents rapamycin, cyclosporin A and tacrolimus increase lipolysis, inhibit lipid storage and alter expression of genes involved in lipid metabolism in human adipose tissue. Cyclosporin A (CsA), tacrolimus and rapamycin are immunosuppressive agents (IAs) associated with insulin resistance and dyslipidemia, although their molecular effects on lipid metabolism in adipose tissue are unknown. We explored IAs effects on lipolysis, lipid storage and expression of genes involved on lipid metabolism in isolated human adipocytes and/or adipose tissue obtained via subcutaneous . https://doi.org/10.1016/j.mce.2012.10.030
Aureliano, M.; Fraqueza, G.; Ohlin, C.A.. 2013. "Ion pumps as biological targets for decavanadate". Dalton Transactions, 42 (33): 11770-11777. https://doi.org/10.1039/c3dt50462j
Ramos, S.; Moura, J.J.G.; Aureliano, M.. 2013. A comparison between vanadyl, vanadate and decavanadate effects in actin structure and function: Combination of several spectroscopic studies. Vol. 7. https://doi.org/10.3233/978-1-61499-184-7-261
Aureliano, M.. 2012. A comparison between Vanadyl, Vanadate, and decavanadate effects in actin structure and function: combination of several spectroscopic studies. The studies about the interaction of actin with vanadium are seldom. In the present paper the effects of vanadyl, vanadate, and decavanadate in the actin structure and function were compared. Decavanadate clearly interacts with actin, as shown by 51V-NMR spectroscopy. Decavanadate interaction with actin induces protein cysteine oxidation and vanadyl formation, being both prevented by the natural l. http://hdl.handle.net/10400.1/1449
Fraqueza, G; Batista de Carvalho, LAE; Marques, MPM; Ohlin, CA; Casey, WH; Aureliano, M. 2012. Functional and Structural Interactions of Nb, V, Mo and W Oxometalates with the Sarcoplasmic Reticulum Ca2+-ATPase Reveal New Insights Into Inhibition Processes: a Combination of NMR, Raman, AA and EPR Spectroscopies with Kinetic Studies. http://hdl.handle.net/10316/45091
Pereira, Maria João; Palming, Jenny; Rizell, Magnus; Aureliano, M.; Carvalho, Eugénia; Svensson, Maria K.; Eriksson, Jan W.; et al. 2012. mTOR inhibition with rapamycin cause impaired insulin signalling and glucose uptake in human subcutaneous and omental adipocytes. Rapamycin is an immunosuppressive agent used after organ transplantation, but its molecular effects on glucose metabolism needs further evaluation. We explored rapamycin effects on glucose uptake and insulin signalling proteins in adipocytes obtained via subcutaneous (n = 62) and omental (n = 10) fat biopsies in human donors. At therapeutic concentration (0.01 lM) rapamycin reduced basal and insul. https://doi.org/10.1016/j.mce.2012.01.024
Aureliano, M.; Nolasco, P.A.; Da Silva, J.J.R.F.; Da Silva, J.A.L.. 2012. "Metalloids in origin and evolution of life,Os semimetais na origem e evolução da vida". Quimica Nova, 35 (5): 1062-1068. http://www.scopus.com/inward/record.url?eid=2-s2.0-84864221679&partnerID=MN8TOARS
Fraqueza, Gil; Batista de Carvalho, Luis A E; Marques, M Paula M; Maia, Luisa; Ohlin, C Andre; Casey, William H; Aureliano, Manuel; et al. 2012. "Decavanadate, decaniobate, tungstate and molybdate interactions with sarcoplasmic reticulum Ca(2+)-ATPase: quercetin prevents cysteine oxidation by vanadate but does not reverse ATPase inhibition.". Dalton transactions (Cambridge, England : 2003), 41 (41): 12749-58. https://doi.org/10.1039/c2dt31688a
Fraqueza, G.; Ohlin, C.A.; Casey, W.H.; Aureliano, M.; Fraqueza, Gil; Ohlin, C. André; Casey, William H.. 2012. "Sarcoplasmic reticulum calcium ATPase interactions with decaniobate, decavanadate, vanadate, tungstate and molybdate". Journal of Inorganic Biochemistry, 107 (1): 82-89. https://doi.org/10.1016/j.jinorgbio.2011.10.010
Ramos, S.; Moura, J.J.G.; Aureliano, M.; Ramos, Susana; Moura, José J. G.. 2012. "Recent advances into vanadyl, vanadate and decavanadate interactions with actin". Metallomics, 4 (1): 16-22. https://doi.org/10.1039/c1mt00124h
Ramos, S.; Moura, J.J.G.; Aureliano, M.. 2012. "A comparison between vanadyl, vanadate, and decavanadate effects in actin structure and function: Combination of several spectroscopic studies". Spectroscopy (New York), 27 (5-6): 355-359. https://doi.org/10.1155/2012/532904
Manuel Aureliano; Pedro A. Nolasco; João J. R. Fraústo da Silva; José Armando L. da Silva; Aureliano, M.; Nolasco, Pedro A.; Silva, João J. R. Fraústo da; Silva, José Armando L.. 2012. "Os semimetais na origem e evolução da vida". Química Nova, 35 (5): 1062-1068. https://doi.org/10.1590/s0100-40422012000500036
Matos, Elisabete; Silva, Tomé S.; Tiago, Teresa; Aureliano, M.; Dinis, Maria Teresa; Dias, J.; Matos, E.; et al. 2011. Effect of harvesting stress and storage conditions on protein degradation in fillets of farmed gilthead seabream (Sparus aurata): a differential scanning calorimetry study. A trial was undertaken to evaluate Differential Scanning Calorimetry (DSC) as a fast analytical tool to differentiate gilthead seabream subjected to variable conditions of slaughter stress and post-mortem storage. Fish were subjected to different harvesting stress conditions: profound anaesthesia (PA, low stress) and net crowding (NC, high stress). Fish were slaughtered in an ice-salt water slurry. https://doi.org/10.1016/j.foodchem.2010.11.017
Tiago, Teresa; Marques-da-Silva, Dorinda; Samhan-Arias, A. K.; Aureliano, M.; Gutiérrez-Merino, Carlos; Tiago, T.; Marques-da-Silva, D.; Samhan-Arias, A.K.; Gutierrez-Merino, C.. 2011. Early disruption of the actin cytoskeleton in cultured cerebellar granule neurons exposed to 3-morpholinosydnonimine-oxidative stress is linked to alterations of the cytosolic calcium concentration. Cytoskeleton damage is a frequent feature in neuronal cell death and one of the early events in oxidantinduced cell injury. This work addresses whether actin cytoskeleton reorganization is an early event of SIN-1-induced extracellular nitrosative/oxidative stress in cultured cerebellar granule neurons (CGN). The actin polymerization state, i.e. the relative levels of G-/F-actin, was quantitatively. https://doi.org/10.1016/j.ceca.2011.01.009
Aureliano M; Aureliano, M.. 2011. "Recent perspectives into biochemistry of decavanadate.". World journal of biological chemistry. https://doi.org/10.4331/wjbc.v2.i10.215
Ramos, S.; Almeida, R.M.; Moura, J.J.G.; Aureliano, M.; Ramos, Susana; Almeida, Rui M.; Moura, José J. G.. 2011. "Implications of oxidovanadium(IV) binding to actin". Journal of Inorganic Biochemistry, 105 (6): 777-783. https://doi.org/10.1016/j.jinorgbio.2011.02.010
Tiago, Teresa; Palma, Pedro; Gutiérrez-Merino, Carlos; Aureliano, M.; Tiago, T.; Palma, P.S.; Gutierrez-Merino, C.. 2010. Peroxynitrite-mediated oxidative modifications of myosin and implications on structure and function. The peroxynitrite-induced functional impairment of myosin was studied in different reaction conditions, known to alter the oxidative chemistry of peroxynitrite, to better understand the molecular mechanisms of this interaction. It is shown that peroxynitrite is able to enhance the basal MgATPase activity up to 2-fold while inhibiting the actin-stimulated ATPase activity of myosin and that the exte. https://doi.org/10.3109/10715762.2010.502170
Ramos, Susana; Moura, José J. G.; Aureliano, M.. 2010. Actin as a potential target for decavanadate. ATP prevents G-actin cysteine oxidation and vanadyl formation specifically induced by decavanadate, suggesting that the oxometalate–protein interaction is affected by the nucleotide. The ATP exchange rate is increased by 2-fold due to the presence of decavanadate when compared with control actin (3.1×10-3 s-1), and an apparent dissociation constant (kdapp) of 227.4±25.7 µM and 112.3±8.7 µM was obt. http://hdl.handle.net/10400.1/1325
Ramos, S.; Moura, J.J.G.; Aureliano, M.; Ramos, Susana; Moura, Jose J. G.; Aureliano, Manuel. 2010. "Actin as a potential target for decavanadate". Journal of Inorganic Biochemistry, 104 (12): 1234-1239. https://doi.org/10.1016/j.jinorgbio.2010.08.001
Aureliano, M.. 2009. Decavanadate: a journey in a search of a role. Currently, efforts have been directed towards using decavanadate as a tool for the understanding of several biochemical processes such as muscle contraction, calcium homeostasis, in vivo changes of oxidative stress markers, mitochondrial oxygen consumption, mitochondrial membrane depolarization, actin polymerization and glucose uptake, among others. In addition, studies have been conducted in orde. http://hdl.handle.net/10400.1/1304
Aureliano, M.; Crans, Debbie C.. 2009. Decavavanadate (V10 O6-28) and oxovanadates: oxometalates with many biological activities. The decameric vanadate species V10O6 28 , also referred to as decavanadate, impact proteins, lipid structures and cellular function, and show some effects in vivo on oxidative stress processes and other biological properties. The mode of action of decavanadate in many biochemical systems depends, at least in part, on the charge and size of the species and in some cases competes with the simpler ox. http://hdl.handle.net/10400.1/1328
Laizé, Vincent; Tiago, Daniel M.; Aureliano, M.; Cancela, Leonor; Laizé, V.; Tiago, D.M.; Cancela, M.L.. 2009. New insights into mineralogenic effects of vanadate. Vanadium is a transition metal that occurs naturally in a variety of minerals and exhibits an exceptional complex chemistry in solution, e.g., several oxidation states ranging from ?2 to ?5, and formation of vanadium oligomers such as decameric vanadate (?5) species [1–4]. Besides its metallurgical role in steel alloys, vanadium is also an ultra trace element known to participate in many biologica. https://doi.org/10.1007/s00018-009-0137-9
Ramos, Susana; Duarte, Rui O.; Moura, José J. G.; Aureliano, M.. 2009. Decavanadate interactions with actin: cysteine oxidation and vanadyl formation. Incubation of actin with decavanadate induces cysteine oxidation and oxidovanadium(IV) formation. The studies were performed combining kinetic with spectroscopic (NMR and EPR) methodologies. Although decavanadate is converted to labile oxovanadates, the rate of deoligomerization can be very slow (half-life time of 5.4 h, at 25 ¿C, with a first order kinetics), which effectively allows decavanadate. http://hdl.handle.net/10400.1/1357
Pereira, Maria João; Carvalho, Eugénia; Eriksson, Jan W.; Crans, Debbie C.; Aureliano, M.; Pereira, M.J.; Carvalho, E.; Eriksson, J.W.; Crans, D.C.. 2009. Effects of decavanadate and insulin enhancing vanadium compounds on glucose uptake in isolated rat adipocytes. The effects of different vanadium compounds namely pyridine-2,6-dicarboxylatedioxovanadium(V) (V5-dipic), bis(maltolato) oxovanadium(IV) (BMOV) and amavadine, and oligovanadates namely metavanadate and decavanadate were analysed on basal and insulin stimulated glucose uptake in rat adipocytes. Decavanadate (50 lM), manifest a higher increases (6-fold) on glucose uptake compared with basal, followe. https://doi.org/10.1016/j.jinorgbio.2009.09.015
Aureliano, M.. 2009. "Decavanadate: A journey in a search of a role". Dalton Transactions, (42): 9093-9100. https://doi.org/10.1039/b907581j
Ramos, S.; Duarte, R.O.; Moura, J.J.G.; Aureliano, M.. 2009. "Decavanadate interactions with actin: Cysteine oxidation and vanadyl formation". Dalton Transactions, (38): 7985-7994. https://doi.org/10.1039/b906255f
Aureliano, M.; Crans, D.C.. 2009. "Decavanadate (V<inf>10</inf> O<inf>28</inf><sup>6 -</sup>) and oxovanadates: Oxometalates with many biological activities". Journal of Inorganic Biochemistry, 103 (4): 536-546. https://doi.org/10.1016/j.jinorgbio.2008.11.010
Tiago, T.; Silva, D.; Samhan-Arias, A. K.; Aureliano, M.; Gutierrez-Merino, C.. 2009. Actin cytoskeleton disruption is an early event upon exposure of cerebellar granule neurons to SIN-1-induced oxidative stress. Editado por Caporossi, D.; Pigozzi, F.; Sabatini, S..
Aureliano, M.; Henao, Fernando; Tiago, Teresa; Duarte, Rui O.; Moura, José J. G.; Baruah, Bharat; Crans, Debbie C.; et al. 2008. Sarcoplasmic reticulum calcium ATPase is inhibited by organic vanadium coordination compounds: pyridine-2-6-dicarboxylatodioxovanadium(V), BMOV and an amavadine analogue. The general affinity of the sarcoplasmic reticulum (SR) Ca2+-ATPase was examined for three different classes of vanadium coordination complexes including a vanadium(V) compound, pyridine-2,6-dicarboxylatodioxovanadium(V) (PDC-V(V)), and two vanadium(IV) compounds, bis(maltolato)oxovanadium(IV) (BMOV), and an analogue of amavadine, bis(N-hydroxylamidoiminodiacetato)vanadium(IV) (HAIDA-V(IV)). The a. https://doi.org/10.1021/ic702405d
Soares, S. S.; Martins, H.; Gutiérrez-Merino, Carlos; Aureliano, M.. 2008. Vanadium and cadmium in vivo effects in cardiac muscle: metal accumulation and oxidative stress markers. Several biological studies associate vanadium and cadmium with the production of reactive oxygen species (ROS), leading to lipid peroxidation and antioxidant enzymes alterations. The present study aims to analyse and compare the oxidative stress responses induced by an acute intravenous exposure (1 and 7 days) to a sub-lethal concentration (5 mM) of two vanadium solutions, containing different van. http://hdl.handle.net/10400.1/1294
Tiago, D.M.; Cancela, M.L.; Aureliano, M.; Laizé, V.; Tiago, Daniel M.; Cancela, Leonor; Laizé, Vincent. 2008. "Vanadate proliferative and anti-mineralogenic effects are mediated by MAPK and PI-3K/Ras/Erk pathways in a fish chondrocyte cell line". FEBS Letters, 582 (9): 1381-1385. https://doi.org/10.1016/j.febslet.2008.03.025
Tiago, D.M.; Laizé, V.; Cancela, M.L.; Aureliano, M.; Tiago, Daniel M.; Laizé, Vincent; Cancela, Leonor. 2008. "Impairment of mineralization by metavanadate and decavanadate solutions in a fish bone-derived cell line". Cell Biology and Toxicology, 24 (3): 253-263. https://doi.org/10.1007/s10565-007-9034-x
Soares, S.S.; Martins, H.; Gutiérrez-Merino, C.; Aureliano, M.. 2008. "Vanadium and cadmium in vivo effects in teleost cardiac muscle: Metal accumulation and oxidative stress markers". Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, 147 (2): 168-178. https://doi.org/10.1016/j.cbpc.2007.09.003
Soares, S.S.; Henao, F.; Aureliano, M.; Gutiérrez-Merino, C.; Soares, S. S.; Henao, Fernando; Gutiérrez-Merino, Carlos. 2008. "Vanadate induces necrotic death in neonatal rat cardiomyocytes through mitochondrial membrane depolarization". Chemical Research in Toxicology, 21 (3): 607-618. https://doi.org/10.1021/tx700204r
Aureliano, M.; Soares, S. S.; Henao, Fernando; Gutiérrez-Merino, Carlos. 2007. "The pathways of cell death in cardiomyocytes induced by vanadate".
Soares, S. S.; Gutiérrez-Merino, Carlos; Aureliano, M.; Soares, S.S.; Gutiérrez-Merino, C.. 2007. Decavanadate induces mitochondrial membrane depolarization and inhibits oxygen consumption. Decavanadate induced rat liver mitochondrial depolarization at very low concentrations, half-depolarization with 39 nM decavanadate, while it was needed a 130-fold higher concentration of monomeric vanadate (5 lM) to induce the same effect. Decavanadate also inhibits mitochondrial repolarization induced by reduced glutathione in vitro, with an inhibition constant of 1 lM, whereas no effect was obs. https://doi.org/10.1016/j.jinorgbio.2007.01.012
Tiago, Teresa; Martel, Paulo; Gutiérrez-Merino, Carlos; Aureliano, M.; Gutierrez-Merino, Carlos; Aureliano, Manuel. 2007. Binding modes of decavanadate to myosin and inhibition of the actomyosin ATPase activity. Decavanadate, a vanadate oligomer, is known to interact with myosin and to inhibit the ATPase activity, but the putative binding sites and the mechanism of inhibition are still to be clarified. We have previously proposed that the decavanadate (V10O28 6-) inhibition of the actin-stimulated myosin ATPase activity is non-competitive towards both actin and ATP. A likely explanation for these results . https://doi.org/10.1016/j.bbapap.2007.02.004
Aureliano, M.. 2007. Vanadium biochemistry. Portugal: Research SignPost. http://hdl.handle.net/10400.1/1260
Manuel Aureliano; Sandra S. Soares; Teresa Tiago; Susana Ramos; Carlos Gutiérrez-Merino. 2007. Biological Effects of Decavanadate: Muscle Contraction, In Vivo Oxidative Stress, and Mitochondrial Toxicity. https://doi.org/10.1021/bk-2007-0974.ch018
M. Aureliano. 2007. "Effects of decavanadate in cardiac myocytes".
M. Aureliano. 2007. "Decavanadate toxicity".
Aureliano, M.; Soares, S.S.; Tiago, T.; Ramos, S.; Gutiérrez-Merino, C.. 2007. "Biological effects of decavanadate: Muscle contraction, in vivo oxidative stress, and mitochondrial toxicity". ACS Symposium Series, 974: 249-263. http://www.scopus.com/inward/record.url?eid=2-s2.0-36749016168&amp;partnerID=MN8TOARS
Soares, Sandra S.; Gutierrez-Merino, Carlos; Aureliano, Manuel; Soares, S. S.; Gutiérrez-Merino, Carlos; Aureliano, M.. 2007. "Mitochondria as a target for decavanadate toxicity in Sparus aurata heart". Aquatic Toxicology, 83 (1): 1-9. https://doi.org/10.1016/j.aquatox.2007.03.005
Soares, S.S.; Martins, H.; Duarte, R.O.; Moura, J.J.G.; Coucelo, J.; Gutiérrez-Merino, C.; Aureliano, M.; et al. 2007. "Vanadium distribution, lipid peroxidation and oxidative stress markers upon decavanadate in vivo administration". Journal of Inorganic Biochemistry, 101 (1): 80-88. https://doi.org/10.1016/j.jinorgbio.2006.08.002
Tiago, Teresa; Ramos, Susana; Aureliano, M.; Gutiérrez-Merino, Carlos; Tiago, T; Ramos, S; Aureliano, M; Gutierrez-Merino, C. 2006. Peroxynitrite induces F-actin depolymerization and blockade of myosin ATPase stimulation. Treatment of F-actin with the peroxynitrite-releasing agent 3-morpholinosydnonimine (SIN-1) produced a dose-dependent F-actin depolymerization. This is due to released peroxynitrite because it is not produced by ‘decomposed SIN-1’, and it is prevented by superoxide dismutase concentrations efficiently preventing peroxynitrite formation. F-actin depolymerization has been found to be very sensitive . https://doi.org/10.1016/j.bbrc.2006.01.112
Ramos, Susana; Manuel, Miguel; Tiago, Teresa; Duarte, Rui O.; Martins, Jorge; Gutiérrez-Merino, Carlos; Moura, José J. G.; Aureliano, M.. 2006. Decavanadate interactions with actin: inhibition of G-actin polymerization and stabilization of decameric vanadate. Decameric vanadate species (V10) inhibit the rate and the extent of G-actin polymerization with an IC50 of 68 ± 22 lM and 17 ± 2 lM, respectively, whilst they induce F-actin depolymerization at a lower extent. On contrary, no effect on actin polymerization and depolymerization was detected for 2 mM concentration of ‘‘metavanadate’’ solution that contains ortho and metavanadate species, as observed. https://doi.org/10.1016/j.jinorgbio.2006.06.007
Soares, S. S.; Martins, H.; Aureliano, M.; Soares, SS; Martins, H; Aureliano, M. 2006. Vanadium distribution following decavanadate administration. An acute exposure of two vanadate solutions— metavanadate and decavanadate—containing different vanadate oligomers, induces different patterns of subcellular vanadium distribution in blood plasma, red blood cells (RBC), and cardiac muscle subcellular fractions of the fish Sparus aurata (gilthead seabream). The highest amount of vanadium was found in blood plasma 1 h after (5 mM) intravenous vanada. https://doi.org/10.1007/s00244-004-0246-2
Tiago, T.; Simao, S.; Aureliano, M.; Martin-Romero, F. J.; Gutierrez-Merino, C.; Tiago, Teresa; Simão, Sónia; Martín-Romero, Francisco Javier; Gutiérrez-Merino, Carlos. 2006. "Inhibition of skeletal muscle S1-myosin ATPase by peroxynitrite". Biochemistry, 45 (11): 3794-3804. https://doi.org/10.1021/bi0518500
Aureliano, M.; Gândara, Ricardo M. C.. 2005. Decavanadate effects in biological systems. Vanadium biological studies often disregarded the formation of decameric vanadate species known to interact, in vitro, with high-affinity with many proteins such as myosin and sarcoplasmic reticulum calcium pump and also to inhibit these biochemical systems involved in energy transduction. Moreover, very few in vivo animal studies involving vanadium consider the contribution of decavanadate to van. https://doi.org/10.1016/j.jinorgbio.2005.02.024
Gândara, Ricardo M. C.; Soares, S. S.; Martins, H.; Gutiérrez-Merino, Carlos; Aureliano, M.. 2005. Vanadate oligomers: in vivo effects in hepatic vanadium accumulation and stress markers. The formation of vanadate oligomeric species is often disregarded in studies on vanadate effects in biological systems, particularly in vivo, even though they may interact with high affinity with many proteins. We report the effects in fish hepatic tissue of an acute intravenous exposure (12, 24 h and 7 days) to two vanadium(V) solutions, metavanadate and decavanadate, containing different vanadat. http://hdl.handle.net/10400.1/1295
Lima, Ana M. S.; Neves, Margarida R.; Cordeiro, Clara; Aureliano, M.. 2005. O conceito de cientista em diferentes níveis de ensino. O ensino das ciências experimentais envolve um grande empenho, além de um consumo de tempo elevado, da parte dos professores para promover e induzir uma aprendizagem dinâmica de diversos conceitos científicos. Neste trabalho, é analisado o conceito de cientista no ensino básico e universitário. O presente estudo, relativo ao ano lectivo 2004 / 05, envolveu cerca de 600 alunos do ensino básico, des. http://hdl.handle.net/10400.1/1305
Aureliano, M.; Tiago, T.; Gândara, R.M.C.; Sousa, A.; Moderno, A.; Kaliva, M.; Salifoglou, A.; et al. 2005. "Interactions of vanadium(V)-citrate complexes with the sarcoplasmic reticulum calcium pump". Journal of Inorganic Biochemistry, 99 (12): 2355-2361. https://doi.org/10.1016/j.jinorgbio.2005.09.002
Gândara, R.M.C.; Soares, S.S.; Martins, H.; Gutiérrez-Merino, C.; Aureliano, M.. 2005. "Vanadate oligomers: In vivo effects in hepatic vanadium accumulation and stress markers". Journal of Inorganic Biochemistry, 99 (5): 1238-1244. https://doi.org/10.1016/j.jinorgbio.2005.02.023
Tiago, Teresa; Aureliano, M.; Moura, José J. G.; Tiago, T.; Moura, J.J.G.. 2004. Decavanadate as a biochemical tool in the elucidation of muscle contraction regulation. Recently reported decameric vanadate (V10) high affinity binding site in myosin S1, suggests that it can be used as a tool in the muscle contraction regulation. In the present article, it is shown that V10 species induces myosin S1 cleavage, upon irradiation, at the 23 and 74 kDa sites, the latter being prevented by actin and the former blocked by the presence of ATP. Identical cleavage patterns w. https://doi.org/10.1016/j.jinorgbio.2004.08.013
Tiago, T.; Aureliano, M.; Gutierrez-Merino, C.; Tiago, Teresa; Gutiérrez-Merino, Carlos. 2004. "Decavanadate binding to a high affinity site dear the myosin catalytic centre inhibits F-actin-stimulated myosin ATPase activity". Biochemistry, 43 (18): 5551-5561. https://doi.org/10.1021/bi049910+
Soares, S.S.; Aureliano, M.; Joaquim, N.; Coucelo, J.M.. 2003. "Cadmium and vanadate oligomers effects on methaemoglobin reductase activity from Lusitanian toadfish: In vivo and in vitro studies". Journal of Inorganic Biochemistry, 94 (3): 285-290. https://doi.org/10.1016/S0162-0134(03)00006-0
Aureliano, M.. 2002. Quenching of myosin intrinsic fluorescence unravels the existence of a high affinity binding site for decavanadate. Decavanadate, one of the aggregated species of vanadate, is a potent inhibitor of several enzymes, includingskeletalmusclemyosin.However,itsputativebindingsitesinmyosinarelargelyunknown. Titration of the intrinsic fluorescence of myosin, purified from rabbit skeletal muscle, have been carried out in 0.3 M KCl, 5 mM CaCl2 and 25 mM Tris-HCl (pH 7.0), with 0.1 mg/ml myosin. In the 0–200¿M total vana. http://hdl.handle.net/10400.1/4809
Tiago, T.; Aureliano, M.; Gutiérrez-Merino, C.. 2002. "Quenching of Myosin Intrinsic Fluorescence Unravels the Existence of a High Affinity Binding Site for Decavanadate". Journal of Fluorescence, 12 (1): 87-90. https://doi.org/10.1023/A:1015371422083
Aureliano, M.; Joaquim, N.; Sousa, A.; Martins, H.; Coucelo, J.M.. 2002. "Oxidative stress in toadfish (Halobactrachus didactylus) cardiac muscle - Acute exposure to vanadate oligomers". Journal of Inorganic Biochemistry, 90 (3-4): 159-165. https://doi.org/10.1016/S0162-0134(02)00414-2
Aureliano, M.. 2000. Vanadate oligomers interactions with myosin. ‘Monovanadate’containingamixtureofatleastfourdifferentvanadatespeciesand‘decavanadate’containingapparentlyonlytwovanadate species, mainly decameric species, inhibit myosin and actomyosin ATPase activities. The addition of myosin to ‘monovanadate’ and ‘decavanadate’solutionspromotesdifferentialincreasesonthe 51VNMRspectrallinewidthsofvanadateoligomers.Therelativeorderofline broadening upon myosin a. https://doi.org/10.1016/S0162-0134(00)00021-0
Aureliano, M.; Manuel Aureliano. 2000. Vanadate oligomer inhibition of passive and active Ca2+ translocation by the Ca2+ pump of sarcoplasmic reticulum. ‘Monovanadate’ containing mainly monomeric, dimeric and tetrameric vanadate species or ‘decavanadate’, containingmainlydecameric vanadate species inhibits the passive and the active ef¿ux of Ca2+ through the sarcoplasmic reticulum calcium pump. When the ef¿ux of Ca2+ by sarcoplasmic reticulum vesicles is not associated with ATP synthesis both vanadate solutions inhibit the passive ef¿ux of Ca 2+. . https://doi.org/10.1016/s0162-0134(00)00022-2
Aureliano, M.; Pedroso, M.C.; De Lima; Carvalho, A.P.; Pires, E.M.V.. 1995. "Effect of myosin phosphorylation on actomyosin ATPase activity: a flow microcalorimetric study". Thermochimica Acta, 258 (C): 59-66. https://doi.org/10.1016/0040-6031(94)02192-Q
Aureliano, M.; Leta, J.; Madeira, V. M. C.; Demeis, L.. 1994. "THE CLEAVAGE OF PHOSPHOENOLPYRUVATE BY VANADATE". Biochemical and Biophysical Research Communications, 201 (1): 155-159. https://doi.org/10.1006/bbrc.1994.1682
Aureliano, M.; Madeira, V. M. C.. 1994. "INTERACTIONS OF VANADATE OLIGOMERS WITH SARCOPLASMIC-RETICULUM CA2+-ATPASE". Biochimica Et Biophysica Acta-Molecular Cell Research, 1221 (3): 259-271. https://doi.org/10.1016/0167-4889(94)90249-6
Aureliano, M.; Madeira, V. M. C.. 1994. "VANADATE OLIGOANIONS INTERACT WITH THE PROTON EJECTION BY THE CA2+ PUMP OF SARCOPLASMIC-RETICULUM". Biochemical and Biophysical Research Communications, 205 (1): 161-167. https://doi.org/10.1006/bbrc.1994.2644
Amado, A. M.; Aureliano, M.; Ribeiroclaro, P. J. A.; Teixeiradias, J. J.. 1993. "COMBINED RAMAN AND V-51 NMR SPECTROSCOPIC STUDY OF VANADIUM (V) OLIGOMERIZATION IN AQUEOUS ALKALINE-SOLUTIONS". Journal of Raman Spectroscopy, 24 (10): 699-703. https://doi.org/10.1002/jrs.1250241011
Geraldes, Cfgc; Castro, Mmca; Saraiva, M. E.; Aureliano, M.; Dias, B. A.. 1988. "INTERACTION OF MONOSACCHARIDES AND RELATED-COMPOUNDS WITH OXOCATIONS OF MO(VI), W(VI) AND U(VI) STUDIED BY NMR-SPECTROSCOPY". Journal of Coordination Chemistry, 17 (3): 205-219. https://doi.org/10.1080/00958978808070771
 

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