Silhueta de pessoa indiferenciada

Márcio Alexandre Filipe Simão

Professor auxiliar convidado
Faculdade de Medicina e Ciências Biomédicas
Subsistema
Docentes Universitário
Unidade ID externa
Centro de Ciências do Mar do Algarve (CCMAR)
Regime
Parcial
Vínculo
CT em Funções Públicas a termo resolutivo certo
I have a Biochemistry degree (PreBolonha) and a specialization in Molecular Biology with a Master in Molecular Biology and Microbiology obtained at the University of Algarve (UALG). From 2013 to 2017, I was enrolled in the PhD programme in Biomedical Sciences in UALG. I have initiated my scientific career with a fellowship in a biogeochemistry project about oxygen dynamic coupled to organic carbon in intertidal areas, where I have optimized and setup methods for chemical analysis of interstitial waters for several elements (Fe, Zn, NH4, Cd, P) and organic matter, besides sample collections at intertidal sites, I was also responsible for lab manager activities related with the ordering of lab equipment and reagents. At the same time, I worked in my graduation thesis under supervision of Prof. Leonor Cancela (BIOSKEL Lab, at UALG), Prof. Carlos Rocha (Biogeochemistry lab, at UALG), and Dr Ricardo Leite (BIOSKEL Lab, at UALG), where we have established a microcosms system to analyse changes in bioturbation of iron biogeochemistry and in molecular response of the clam Ruditapes decussates upon Perkinsus olseni Infection”. Besides iron biogeochemistry analysis, I have gained experience in several methods for UV/VIS quantification of dissolved elements and molecular biology techniques. Between graduation and master I worked mainly in two different projects, one about Non-invasive monitoring of bone tissue growth in culture using polymer functionalized microelectrode arrays, with Prof. Henrique Gomes (Opto-Electronics lab, CEOT, at UALG), where it was possible to gain experience in in vitro applications using bone derived cell cultures, which was followed by a fellowship in a project coordinated by Dr Ricardo Leite and Prof. Leonor Cancela (both from BIOSKEL Lab, at UALG) and related with the identification and characterization of genes involved in synthesis of isoprenoids in parasite Perkinus olseni. Meanwhile I started my master thesis under the supervision of Dr. Ricardo Leite and Prof. Leonor Cancela, entitled “Cloning, expression and characterization of ferritin gene isoforms in clam Ruditapes decussatus. During this period, I have gained considerable experience in methods of molecular biology like PCR, qPCR, DNA cloning, protein expression and bacteria transformation methods. I have also used several bioinformatic tools for the characterization of newly identified gene transcripts. My PhD project was entitled “Contribution to the molecular characterization of osteoarthritis and osteoporosis phenotypes associated with Hereditary Hemochromatosis” and was done under the supervision of Prof. Leonor Cancela (BIOSKEL lab, at UALG), Prof. Ea Hang Korng (Inserm U1132, BIOSCAR at Hôpital Lariboisière, service de Rhumatologie, Université Paris 7 Denis Diderot, Paris, France) and Prof. Graça Porto (I3S research center at University of Porto, Portugal), which is related with the study of osteoarthritis and osteoporosis associated to hereditary hemochromatosis using mice models for the molecular characterization of musculoskeletal complications. In the last years I have been accumulating experience in research of osteoarthritis and osteoporosis associated to iron overload phenotypes with knowhow in several histological approaches for the characterization of cartilage and bone tissues and micro computerized tomography (micro-CT) technique for bone histomorphometric analysis. I have also solid experience in the development of primary cultures of osteoblasts and chondrocytes and in molecular biology techniques. Part of this work was done also at INSERM U1132 group, BIOSCAR at Hôpital Lariboisière, Paris, France.

Atividades

Atividades
2021/09/15 - 2022/09/30. Orientação. Characterization of ZFP36L1 gene expression associated with osteoarthritis and osteoporosis pathogenesis mechanisms.Biomedical Sciences-disease mechanisms (Mestrado). Orientador de Mafalda Lázaro.
2022/04/01 - 2022/07/20. Orientação. The impact of excess iron on the onset of Alzheimer's disease.Ciências Biomédicas (Licenciatura/Bacharelato). Orientador de André Ferreira.
2022/04/01 - 2022/07/20. Orientação. Validation of the functional activity of upstream ORFs present in the transcripts of bone morphogenetic protein receptor 1A.Ciências Biomédicas (Licenciatura/Bacharelato). Orientador de MargaridaMachado.
2019/02/01 - 2021/12/31. Curso / Disciplina lecionado. Cell differentiation and microenvironments. Oncobiology (Mestrado). Universidade do Algarve Departamento de Ciências Biomédicas e Medicina.
2021/10/15. Apresentação oral de trabalho. Lab-it is taking molecular genetics to school. XXI National Congress of Biochemistry . University of Évora (Évora, Portugal).
2020/09/15 - 2021/10/10. Curso / Disciplina lecionado. Laboratories in Biomedicine. Master in Biomedical Sciences-Disease mechanism (Mestrado). Universidade do Algarve - Campus de Gambelas; Universidade do Algarve Departamento de Ciências Biomédicas e Medicina.
2018/09/15 - 2021/09/15. Curso / Disciplina lecionado. Cell Biology . Biomedical Sciences. Universidade do Algarve Departamento de Ciências Biomédicas e Medicina.
2019/06/01 - 2020/09/01. Orientação. Characterization of 5’ and 3’ UTRs from Bone morphogenetic protein receptor type 1A (Bmpr1A) transcripts under the context of bone metabolism.Molecular Biology and Microbiology (Mestrado). Orientador.
2018/09/15 - 2020/09/01. Curso / Disciplina lecionado. Molecular and Celular Biology Techniques. Biomedical Sciences Master (Mestrado). Universidade do Algarve Departamento de Ciências Biomédicas e Medicina.
2019/10/01 - 2020/05/01. Orientação. Identification and characterization and phylogeny of Bmpr1aa and Bmpr1ab 3’ UTR alternative transcripts in Danio rerio.Biomedical Sciences (Licenciatura/Bacharelato). Orientador de Manuel Cerqueira.
2019/10/31. Apresentação oral de trabalho. HFE loss of function is a susceptibility factor for bone loss and early osteoporosis onset. 11th Symposium in Metabolism, "Ageing & Metabolism", Porto. Universidade do Porto, faculdade de Medicina (Porto, Portugal).
2019/02/01 - 2019/07/20. Orientação. Espondilite Anquilosante: Identificação de polimorfismos no alelo HLA-B27 e possíveis suscetibilidades dos hábitos alimentares.Biomedical Sciences (Licenciatura/Bacharelato). Orientador de Daniela Felício.
2018/03/01 - 2018/07/05. Orientação. Associação entre Osteoartrose, Obesidade e Metabolismo do ferro, um estudo exploratório.Biomedical Sciences (Licenciatura/Bacharelato). Coorientador.
2018/03/16. Apresentação oral de trabalho. Characterization of osteoarthritis associated with Hereditary Hemochromatosis using Hfe-KO mouse e model. II Jornadas do Algarve Biomedical Center (ABC). Algarve Biomedical Center (ABC), Universidade do Algarve (Olhão, Portugal).
2017/02/01 - 2017/07/15. Orientação. Abordagem morfológica e molecular ao estudo de factores de desenvolvimento da osteoporose.Biomedical Sciences (Licenciatura/Bacharelato). Coorientador de Xavier Guerreiro.
2016/02/01 - 2016/07/15. Orientação. Caracterização da expressão do transportador de Zinco ZIP14 no peixe-zebra (Danio rerio) e sua validação para estudos biomédicos.Biomedical Sciences (Licenciatura/Bacharelato). Coorientador de Beatriz Estremores.
2015/02/01 - 2015/07/10. Orientação. Caracterização de uma cultura de osteoblastos primária isolada a partir de ratinhos Hfe KO. Impacto do excesso de ferro na mineralização.Biomedical sciences (Licenciatura/Bacharelato). Coorientador de Gabriela Carrasqueira.
2014/10/23. Apresentação oral de trabalho. Characterization of The Histological And Morphological Profile Of Articular Damage In A Hemochromatosis Mouse Model. 34th Congress of the Portuguese Society of Orthopaedics and Traumatology in Algarve . Portuguese Society of Orthopaedics and Traumatology (Albufeira, Portugal).
2014/07/21. Apresentação oral de trabalho. CSI and Prenatal Diagnosis to Teach Molecular Biology: Lab-it – itinerant laboratory. CSI and Prenatal Diagnosis to Teach Molecular Biology: Lab-it – itinerant laboratory. HSCI’2014: Science Education with and for Society (Aveiro, Portugal).
2008/07/06. Apresentação oral de trabalho. Non-invasive monitoring of bone tissue growth in culture using polymer functionalized microelectrode arrays. International Conference on Science and Tecnology of Syntehetic Metals (ICSM). Science and Technology of Synthetic Metals (Porto de Galinhas, Pernambuco, Brasil).

Projetos

Projetos
2019/11/01 - 2021/12/31. KCITAR ALGARVE, CRESC Algarve 2020 788.289,50€. Outra. Universidade do Algarve.
2019/03/01 - 2019/05/31. Reversing the pathophysiology of Perkinsosis in clams hatcheries through the use of analogues of Artemisinin, Projectos de I&D FCT. Bolseiro de Pós-Doutoramento. Fundação Calouste Gulbenkian.
2012/02/01 - 2017/05/24. Contribution to the molecular characterization of osteoarthritis and osteoporosis phenotypes associated with Hereditary hemochromatosis, Bolsas de Formação Avançada- PhD fellowships. Bolseiro de Doutoramento. Centro de Ciências do Mar; Universidade do Algarve Departamento de Ciências Biomédicas e Medicina.
2010/02/01 - 2012/01/31. Laboratório itinerante de Genética Molecular (Lab-IT), Laboratório itinerante de Genética Molecular (Lab-IT). Bolseiro de Investigação. Universidade do Algarve - Campus de Gambelas.
2009/04/02 - 2009/11/12. PTARGET- Characterization of isoprenoids biosynthesis and determination of potential therapeutic targets, Projectos de I&D FCT. Bolseiro de Técnico de Investigação. Centro de Ciências do Mar.
2007/11/01 - 2008/01/31. Premio Ceratónia/CGD 2006 prémio na área científica de Telecomunicações e Electrónica “Microelectronic circuits to monitor cells” 25K€, Premio Ceratónia. Bolseiro de Investigação. Centro de Electronica Optoelectrônica e Telecomunicacoes.
2004/04/02 - 2005/04/20. Oxygen dynamic coupled with organic carbon mineralization in inter-tidal sandy zones. Bolseiro de Iniciação Científica. Universidade do Algarve Centro de Investigação Marinha e Ambiental.

Produções

Simão, Márcio; Conceição, Natércia; Imaginário, Susana; Amaro, João; Leonor Cancela. Autor correspondente: Simão, Márcio. 2022. "Lab-It Is Taking Molecular Genetics to School". BioChem, 2: 160-170. https://www.mdpi.com/2673-6411/2/2/11#cite
Márcio Simão; Natércia Conceição; Susana Imaginário; João Amaro; Maria Leonor Cancela. 2022. "Lab-It Is Taking Molecular Genetics to School". BioChem. https://doi.org/10.3390/biochem2020011
Márcio Simão; M. Leonor Cancela. 2021. "Musculoskeletal complications associated with pathological iron toxicity and its molecular mechanisms". Biochemical Society Transactions. https://doi.org/10.1042/BST20200672
Felício, Daniela; Camacho, António; Cancela, M. Leonor; Simão, Márcio. 2020. Identification and characterization of polymorphisms in HLA-B*27 allele in ankylosing spondylitis patients. Em International Meeting of the Portuguese Society of Genetics .
Silva, Joel; Cerqueira, Manuel; Simão, Márcio; Cancela, M. Leonor. 2020. Characterization of 5' and 3' UTRs from Bone morphogenetic protein receptor type 1A (Bmpr1A) transcripts under the context of bone metabolism. Em International Meeting of the Portuguese Society of Genetics.
M. Kyla Shea; Sarah L. Booth; Stephanie G. Harshman; Donald Smith; Cathy S. Carlson; Lindsey Harper; Alexandra R. Armstrong; et al. 2020. "The effect of vitamin K insufficiency on histological and structural properties of knee joints in aging mice". Osteoarthritis and Cartilage Open. https://doi.org/10.1016/j.ocarto.2020.100078
M. Kyla Shea; Stephanie G. Harshman; Sarah L. Booth; Donald Smith; Cathy S. Carlson; Lindsey Harper; M. Leonor, Cancela; Simão, Márcio; Richard F. Loeser. 2020. The effect of vitamin K insufficiency on the development of osteoarthritis in aging mice. Em American Society of Nutrition meeting -NUTRITION 2020 LIVE ONLINE .
Márcio Simão; Ricardo B. Leite; M. Leonor Cancela. 2020. "Expression of four new ferritins from grooved carpet shell clam Ruditapes decussatus challenged with Perkinsus olseni and metals (Cd, Cu and Zn)". Aquatic Toxicology, 105675-105675. https://doi.org/10.1016/j.aquatox.2020.105675
Silva, Joel M.; Simão, Márcio; Cancela, M. Leonor. 2020. Molecular characterization of regulatory elements in the 5'- and 3'- UTRs of Bmpr1a transcripts. Em Annual Meeting of the Portuguese Society of Human Genetics.
Márcio Simão; Paulo J. Gavaia; António Camacho; Graça Porto; I. Jorge Pinto; Hang-Korng Ea; M. Leonor Cancela. 2019. "Intracellular iron uptake is favored in Hfe -KO mouse primary chondrocytes mimicking an osteoarthritis-related phenotype". BioFactors. https://doi.org/10.1002/biof.1520
Felício, Daniela; Camacho, António; Cancela, M. Leonor; Simão, Márcio. 2019. Bioinformatic analysis and characterization of pathogenic mutations on HLA-B27 alleles and its putative implications on protein structure. Em III Jornadas do Algarve Biomedical Center (ABC).
Simão M; Camacho A; Ostertag A; Cohen-Solal M; Pinto IJ; Porto G; Hang Korng E; Cancela ML. 2018. "Iron-enriched diet contributes to early onset of osteoporotic phenotype in a mouse model of hereditary hemochromatosis.". PloS one. https://doi.org/10.1371/journal.pone.0207441
ESTREMORES, BEATRIZ; Simão, Márcio; Gavaia, Paulo J; Cancela, M. Leonor. 2018. Phylogenetic and molecular characterization of Slc39a14 (Zip14) zinc transporter in Danio rerio. Em Interdisciplinary Approaches in Fish Skeletal Biology (IAFSB), 5th Conference.
Simão, Márcio; Camacho, António; Ostertag, Ágnes; Cohen-Solal, Martine; Pinto, I. Jorge; Porto, Graça; Ea, Hang-Korng; Cancela, M. Leonor. 2018. Iron enriched diet in association with Hfe loss of function promote iron toxicity mechanism and accelerate bone loss phenotype. Em Free Radical Biology & Medicine biennial meeting.
Simão, Márcio. 2017. "Contribution to the molecular characterization of osteoarthritis and osteoporosis phenotypes associated with Hereditary hemochromatosis". Doutoramento.
2016. "Iron overload in a murine model of hereditary hemochromatosis is associated with accelerated progression of osteoarthritis under mechanical stress.". Osteoarthritis and cartilage. https://doi.org/10.1007/s00244-010-9490-9
2015. "Effect of C282Y genotype on self-reported musculoskeletal complications in hereditary hemochromatosis.". PloS one. https://doi.org/10.1371/journal.pone.0122817
Simão, Márcio; Pinto, I. Jorge; Porto, Graça; Ea, Hang-Korng; Cohen-Solal, Martine; Cancela, M. Leonor. 2015. Iron enriched diet is a determinant factor for osteoporosis onset and progression in hereditary hemochromatosis mouse model (Hfe-KO). Em 4th Barcelona PhD Students Symposium- Science fights back: Tackling disease to recover homeostasis. .
Simão, Márcio; Gavaia, Paulo J; Ea, Hang-Korng; Pinto, I. Jorge; Cancela, M. Leonor. 2014. Chondrocytes from a hemochromatosis mouse model reveal altered expression of genes associated to iron and cartilage metabolisms. Em XVIII Congress of the Portuguese Biochemical Society.
Carvalho, Filipe R.; Cardeira, João; Simão, Márcio; Gavaia, Paulo J; Cancela, M. Leonor. 2013. Inducing a transient diabetic phenotype in zebrafish: can this model be used to study insulin related changes in bone metabolism?. Em Interdisciplinary Approaches in Fish Skeletal Biology (IAFSB) 4th Conference.
Simão, Márcio; Gavaia, P; Pinto, I. Jorge; Ea, Hang-Korng; Cancela, M. Leonor. 2013. Establishing an in vitro system to study chondrocyte phenotypes associated to human hereditary hemochromatosis and identify molecular players involved in chondrocyte related iron metabolism. Em European Calcified Tissue Society (ECTS) Congress.
Simão, Márcio Alexandre Filipe. 2011. "Identificação e caracterização de duas isoformas da Ferritina da amêijoa R. decussatus e avaliação de algumas das suas propriedades biológicas". http://hdl.handle.net/10400.1/2655
Simão, M.F.; Leite, R.B.; Rocha, C.; Cancela, M.L.. 2010. "Changes in bioturbation of iron biogeochemistry and in molecular response of the clam ruditapes decussates upon perkinsus olseni infection". Archives of Environmental Contamination and Toxicology, 59 (3): 433-443. https://doi.org/10.1016/j.joca.2015.09.007
Simão, Márcio; Leite, Ricardo B.. 2010. Identification and characterization of two Ferritin isoforms in clam R. decussatus and evaluation of their Fe2+ oxidation potential. Em XVII Congresso Nacional de Bioquímica.
Ricardo B. Leite; Luís Fonseca; Ricardo Afonso; Simão, Márcio; Rita Ascenso; M. Leonor Cancela. 2008. Clam Lectins: Species-specific patterns of expression upon perkinus exposure: Evolutionary considerations. Em Final Assembly of Marine Genomics Europe (MGE) .
Ricardo B. Leite; Ricardo Afonso; Simão, Márcio; Sandra Joaquim; Domitília Matias; M. Leonor Cancela. 2007. Evaluation of Perkinsus infection levels within a Variable Range of Temperatures and Salinities in Clam Ruditapes decussatus. Effect on Clam Oxidative Stress and Expression of Antioxidant-related genes. Em WOPER (Workshop for the Analysis of the Impact of Perkinsosis to the European Shellfish Industry) .
Simão, Márcio; Ricardo B. Leite; Ricardo Afonso; Sandra Joaquim; Domitília Matias; M. Leonor Cancela. 2006. Evaluation of Oxidative Stress Levels and Antioxidants mRNA Expression within a Variable Range of Temperatures in Clam Ruditapes decussatus. Em Congresso Nacional de Bioquímica.
Simão, Márcio. 2006. "Molecular response of Ruditapes decussatus clam to Perkinsus olseni parasite infection and its relation with the iron biogeochemistry in inter-tidal sediments.". Licenciatura.
Simão, Márcio; Ricardo B. Leite; M. Leonor Cancela; Carlos Rocha. 2005. Effect of iron dynamics stress on the response of clam R. decussatus to an infection by the protozoan parasite Perkinsus olseni. Em 2nd International Congress on Stress Responses in Biology and Medicine.
Ricardo B. Leite; Ricardo Afonso; Rita Ascenso; Simão, Márcio; M. Leonor Cancela. 2005. Effect of Perkinsus infection on expression of Ruditapes decussatus biomarkers genes in response to environmental pollutants. Em Marine Genomics International Congress.
Simão, Márcio; Cancela; M. Leonor; Leite; Ricardo B.; Rocha; Carlos. 2004. A preliminary study of the effect of benthic iron dynamics on the response of the clam R. decussatus to an infection by the protozoan parasite Perkinsus atlanticus /olseni. Em XIV Congresso Nacional de Bioquímica, Vilamoura .
 

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